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Image Search Results
Journal: Gene Therapy
Article Title: Terminal hairpins improve protein expression in IRES-initiated mRNA in the absence of a cap and polyadenylated tail
doi: 10.1038/s41434-023-00391-4
Figure Lengend Snippet: A Vector schematics. Vectors with triple hairpin structures had slightly longer UTRs. B eGFP expression in HEK293 cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. Differences between hhhRhhh and its capped and polyadenylated variant ChhhRhhhA were not significant, but both outperformed canonical mRNA ( CRA ). C eGFP expression in MDCK cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. D eGFP expression in HEK293 cells 24 h after electroporation with modified mRNA vectors in which all uridines were replaced by N1-Methylpseudouridine (m1Ψ). [h: hairpin; A: long poly(A) tail (AAAAAA); C: Anti-reverse Cap Analog (ARCA); R: main internal segment that includes the eGFP ORF in non-IRES vectors or an IRES and the eGFP ORF] ( n = 4, * P < 0.05 shown for 24 h only).
Article Snippet:
Techniques: Plasmid Preparation, Expressing, Electroporation, Variant Assay, Modification