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TaKaRa star methods pmcherry n1 clontech
Star Methods Pmcherry N1 Clontech, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N1 Methylnicotinamide, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Tokyo Chemical Industry n1-methylnicotinamide m0375—10 g
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Tokyo Chemical Industry xanthurenic acid (xa)
Xanthurenic Acid (Xa), supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cayman Chemical recombinant human nicotinamide-n-methyltransferase (rhnnmt)
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TriLink n 1-methylpseudouridine
A Vector schematics. Vectors with triple hairpin structures had slightly longer UTRs. B eGFP expression in HEK293 cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. Differences between hhhRhhh and its capped and polyadenylated variant ChhhRhhhA were not significant, but both outperformed canonical mRNA ( CRA ). C eGFP expression in MDCK cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. D eGFP expression in HEK293 cells 24 h after electroporation with modified mRNA vectors in which all uridines were replaced by <t>N1-Methylpseudouridine</t> (m1Ψ). [h: hairpin; A: long poly(A) tail (AAAAAA); C: Anti-reverse Cap Analog (ARCA); R: main internal segment that includes the eGFP ORF in non-IRES vectors or an IRES and the eGFP ORF] ( n = 4, * P < 0.05 shown for 24 h only).
N 1 Methylpseudouridine, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TriLink n 1 -methylpseudouridine-5’-triphosphate
A Vector schematics. Vectors with triple hairpin structures had slightly longer UTRs. B eGFP expression in HEK293 cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. Differences between hhhRhhh and its capped and polyadenylated variant ChhhRhhhA were not significant, but both outperformed canonical mRNA ( CRA ). C eGFP expression in MDCK cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. D eGFP expression in HEK293 cells 24 h after electroporation with modified mRNA vectors in which all uridines were replaced by <t>N1-Methylpseudouridine</t> (m1Ψ). [h: hairpin; A: long poly(A) tail (AAAAAA); C: Anti-reverse Cap Analog (ARCA); R: main internal segment that includes the eGFP ORF in non-IRES vectors or an IRES and the eGFP ORF] ( n = 4, * P < 0.05 shown for 24 h only).
N 1 Methylpseudouridine 5’ Triphosphate, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATAGO CO n1 digital refractometer
A Vector schematics. Vectors with triple hairpin structures had slightly longer UTRs. B eGFP expression in HEK293 cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. Differences between hhhRhhh and its capped and polyadenylated variant ChhhRhhhA were not significant, but both outperformed canonical mRNA ( CRA ). C eGFP expression in MDCK cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. D eGFP expression in HEK293 cells 24 h after electroporation with modified mRNA vectors in which all uridines were replaced by <t>N1-Methylpseudouridine</t> (m1Ψ). [h: hairpin; A: long poly(A) tail (AAAAAA); C: Anti-reverse Cap Analog (ARCA); R: main internal segment that includes the eGFP ORF in non-IRES vectors or an IRES and the eGFP ORF] ( n = 4, * P < 0.05 shown for 24 h only).
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Image Search Results


A Vector schematics. Vectors with triple hairpin structures had slightly longer UTRs. B eGFP expression in HEK293 cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. Differences between hhhRhhh and its capped and polyadenylated variant ChhhRhhhA were not significant, but both outperformed canonical mRNA ( CRA ). C eGFP expression in MDCK cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. D eGFP expression in HEK293 cells 24 h after electroporation with modified mRNA vectors in which all uridines were replaced by N1-Methylpseudouridine (m1Ψ). [h: hairpin; A: long poly(A) tail (AAAAAA); C: Anti-reverse Cap Analog (ARCA); R: main internal segment that includes the eGFP ORF in non-IRES vectors or an IRES and the eGFP ORF] ( n = 4, * P < 0.05 shown for 24 h only).

Journal: Gene Therapy

Article Title: Terminal hairpins improve protein expression in IRES-initiated mRNA in the absence of a cap and polyadenylated tail

doi: 10.1038/s41434-023-00391-4

Figure Lengend Snippet: A Vector schematics. Vectors with triple hairpin structures had slightly longer UTRs. B eGFP expression in HEK293 cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. Differences between hhhRhhh and its capped and polyadenylated variant ChhhRhhhA were not significant, but both outperformed canonical mRNA ( CRA ). C eGFP expression in MDCK cells 24 and 48 h after electroporation with equal amounts of the indicated mRNA vectors. D eGFP expression in HEK293 cells 24 h after electroporation with modified mRNA vectors in which all uridines were replaced by N1-Methylpseudouridine (m1Ψ). [h: hairpin; A: long poly(A) tail (AAAAAA); C: Anti-reverse Cap Analog (ARCA); R: main internal segment that includes the eGFP ORF in non-IRES vectors or an IRES and the eGFP ORF] ( n = 4, * P < 0.05 shown for 24 h only).

Article Snippet: N 1 -Methylpseudouridine was from TriLink Biotechnologies (San Diego, CA).

Techniques: Plasmid Preparation, Expressing, Electroporation, Variant Assay, Modification